Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 1;11(12):e0167207. doi: 10.1371/journal.pone.0167207

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Polez et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 3 — (A) Detector traces (Evaporative Light Scattering Detector) from RP-HPLC separation of H-Thr(tBu)-OtBu standard and flow-through to first protein peak fraction from post transpeptidation purification. (B) Detector traces (Evaporative Light Scattering Detector) from RP-HPLC separation of the recovered H-Thr(tBu)-OtBu from transpeptidation reaction of IP. Retention time of H-Thr(tBu)-OtBu is 12.36 min and the purity is 99%. (C) RP-HPLC profile of insulin species after transpeptidation reaction with H-Thr(tBu)-OtBu. (D) RP-HPLC profile of insulin species after transpeptidation reaction with extracted H-Thr(tBu)-OtBu. No difference in efficiency of the transpeptidation reaction was observed.