Fig 2. Response of the pulse detector circuit in Fig 1 for a pulse duration of ½ bacterial cell-cycle (CC) [1020 sec].

The pulse was activated at 10.2 CC (20808 sec) and deactivated at 10.7 CC (21828 sec). The relative strength of RBS1 and RBS2 was 20:1 and the degradation tag had half-life of 4 minutes. The response is average of 20 simulation runs.