Fig 3. Response of the pulse detector circuit in Fig 1 with degradation tags of different half-lifes (2, 4, 8 and 16 minutes).

For brevity only responses of LacZ and CI molecules were displayed for each deg-tag using the same color. The pulse was activated at 10.2 CC (20808 sec) and deactivated at 10.7 CC (21828 sec). The relative strength of RBS1 and RBS2 was 20:1. The response is average of 20 simulation runs.