Fig 5. Specificities and binding affinities of purified chimeric antibodies.

A) ELISA analysis to confirm the specificities of the purified hIgG1 chimeric (upper) and mIgG3 chimeric (lower) anti-EGFR mAbs. Negative control antigens are identical to those described in Fig 4B. B) SPR analysis of anti-EGFR chimeric mAbs. Serially diluted purified chimeric mAbs were applied to the EGFR-Fc immobilized biosensor chip. The KDs were calculated using a bivalent analyte model.