Table 2. Tissue homogenization in ER4 lysis buffer and dilutions for protein and ELISA assays.

Tissue	ER4:tissue homogenization ratio (μl/mg tissue)	Powermax cycles	Protein assay dilution in water	ELISA assay dilution in assay buffer
Brain	10	2 X 5 sec	1:40	1:320
Liver	10	2 X 5 sec	1:40	1:320
Quadriceps	10	2 X 10 sec	1:25	1:40
Spinal cord	10	2 X 5 sec	1:20	1:80