Table 1.
List of Primary Antibodies Used
| No. | Antigen | Catalog No. | Host | Application and Dilution | Vendor |
|---|---|---|---|---|---|
| 1 | GFAP | AB5804 | Rabbit |
IF 1:1000 (tissue culture) |
Millipore |
| 2 | GFP | 13970 | Chicken |
IF 1:1000 (brain sections) |
Abcam |
| 3 | GFP | 598 | Rabbit |
IF 1:1000 (tissue culture) |
MBL |
| 4 | GFP | NB100‐1770 | Goat |
IF 1:500 (tissue culture) |
Novus Biologicals |
| 5 | Lectin FITC | 128K1065 | Mouse |
IF 1:300 (tissue culture) |
Sigma‐Aldrich |
| 6 | MAP2 | M4403 | Mouse |
IF 1:300 (tissue culture) |
Sigma‐Aldrich |
| 7 | MAP2 | 4542 | Rabbit |
IF 1:1000 (tissue culture) |
Cell Signaling Technology |
| 8 | mTOR (7c10) | 2983 | Rabbit | WB 1:500 | Cell Signaling Technology |
| 9 | Nestin | Ab6142 | Mouse |
IF 1:300 (tissue culture) |
Abcam |
| 10 | NeuN | ab104225 | Mouse |
IF 1:1000 (tissue culture) |
Abcam |
| 11 | Oligodendrocytes (RIP) | MAB158 | Mouse |
IF 1:300 (tissue culture) |
Millipore |
| 12 | P‐S6 (Ser235/236) | 4858 | Rabbit |
IF 1:300 (tissue culture); WB 1:500 |
Cell Signaling Technology |
| 13 | P‐S6 (Ser240/Ser244) | 5364 | Rabbit |
IF 1:500 (brain sections) |
Cell Signaling Technology |
| 14 | S6 | 2217 | Rabbit | WB 1:500 | Cell Signaling Technology |
| 15 | P‐Akt (Ser473) | 4060 | Rabbit |
WB 1:1000 IF 1:300 |
Cell Signaling Technology |
| 16 | Akt | 2920 | Mouse | WB 1:1000 | Cell Signaling Technology |
| 17 | Tuj 1 (neuron‐specific class III β‐tubulin) | ab18207 | Mouse |
IF 1:500 (tissue culture) |
Abcam |
| 18 | α‐tubulin | T5168 | Mouse | WB 1:5000 | Sigma‐Aldrich |
IF, immunofluorescence; WB, Western blot.