Table 1. Genes and non-coding regions in the sxt gene cluster with Single Nucleotide polymorphisms compared to A. gracile strain NH-5. Tn = Transition, Tv = Transversion, In = Insertion, Del = Deletion.

Gene	Tn	Tv	In/ Del	Nonsyn	Total	Strain (SNP, InDel, Non-syn) compared to A. gracile strain NH-5
sxtPer	1			1	1	UAM529 (1, -, 1)
sxtE	1			-		NIVA-CYA 655 (1,-,-)
sxtW	1			1	1	NIVA-CYA 851 (1,-,-)
sxtV	3			?	3	NIVA-CYA 851 (3,-,?); NIVA-CYA 655 (1,-?); NIVA-CYA 676 (1,-,?); UAM 529 (1,-,?);
sxtP		1		1	1	NIVA-CYA 851 (1,-,1); NIVA-CYA 655 (1,-,1); NIVA-CYA 676 (1,-,1); UAM 529 (1,-,1)
sxtR	2	2		3	4	NIVA-CYA 655 (4,-,3)
orf24		2		1	2	NIVA-CYA 851 (1,-,-); NIVA-CYA 655 (2,-,1); NIVA-CYA 676 (1,-,-); UAM 529 (1,-,-);
sxtS		1		1	1	NIVA-CYA 655 (1,-,1)
sxtT	1			-	1	NIVA-CYA 676 (1,-,-); UAM 529 (1,-,-);
sxtU		1		-	1	NIVA 851 (1,-,-)
sxtN	1		6	1	2	NIVA-CYA 851 (-,6,-); NIVA-CYA 676 (1,-,1)
sxtG	1			-	1	NIVA-CYA 851 (1,-,-)
sxtH	3	1		3	4	NIVA-CYA 851 (1,-,1); NIVA-CYA 655 (1,-,1); NIVA-CYA 676 (4,-,3); UAM 529 (1,-,1)
sxtI	8	3		4	11	NIVA-CYA 851 (2,-,1); NIVA-CYA 655 (2,-,2); NIVA-CYA 676 (4,-,2) UAM 529 (11,-,4);
sxtL	2	2		2	4	NIVA-CYA 655 (2,-2); NIVA-CYA 676 (3,-1); UAM 529 (3,-,1);
orf3	1	1		2	2	NIVA-CYA 851 (1,-,1); NIVA-CYA 676 (1,-,1); UAM 529 (1,-,1)
Subtotal coding	25	14	6	20	45
sxtA–sxtE	2		126			NIVA-CYA 851 (2,126); NIVA-CYA 676 (1,-); UAM 529 (1,-);
sxtE–sxtW	1					NIVA-CYA 655 (1,-)
sxtV–sxtX			1			NIVA-CYA 851(-,1)
sxtX–sxtD			1			NIVA-CYA 851 (-,1); NIVA-CYA 655 (-,1); NIVA-CYA 676 (-,1) UAM 529 (-,1);
sxtN—sxtG	1					NIVA-CYA 851 (1,-)
sxtL—orf3	1					NIVA-CYA 676 (1,-); UAM 529 (1,-)
Subtotal noncoding	5	0	128	0	133
Total	30	14	134	20	178