Figure 1.

Schematic diagram for alkane production in S. cerevisiae from free fatty acids. An alkane production pathway that utilized a fatty acid α‐dioxygenase (αDOX) for conversion of free fatty acids to aldehydes and subsequent production of alkanes by deformylation of the aldehydes with cyanobacterial aldehyde deformylating oxygenase (cADO) was introduced into S. cerevisiae. The resulting strain can function as a whole‐cell biocatalyst to produce alkane from extracellular fatty acid sources (green path). By deleting FAA1 and FAA4 in S. cerevisiae to allow accumulation of free fatty acid precursors derived from intracellular lipid remodeling and subsequent introduction of the alkane production pathway, a yeast strain for de novo production of alkane from fermentable sugar was developed (blue path).