Skip to main content
. 2016 Mar 14;231(12):2664–2672. doi: 10.1002/jcp.25368

Figure 5.

Figure 5

Proteasome inhibitors prevent DHT‐induced alterations in PPARγ protein. (A) C4‐2 cells were first treated with DMSO vehicle (−) or 10 μM MG132 (+). The cells were then exposed to ethanol vehicle (−) or 1 nM DHT for 24 h. Whole cell lysates were isolated from treated cells, and Western blot analysis was performed to determine the amount of AR, PPARγ and actin protein in each cell extract. (B) C4‐2 cells plated in DHT‐containing media were treated with DMSO, BMS 345541 (10 nM), or bortezomib (0.1 or 1 μM) for 24 h. Western blot analysis was performed on nuclear extracts to measure the level of AR, PPARγ and topoisomerase I protein in treated cells. (C) C4‐2 cells were first transfected with a p65 siRNA SMARTpool siRNA or a non‐targeting control SMARTpool siRNA via electroporation. The cells were then placed in CSS media and treated for 24 h with either EtOH (−) or 1 nM DHT (+). Whole cell lysates were prepared from treated cells. The level of p65 NFκB, PPARγ, AR and actin protein was then measured by Western blot analysis. A representative experiment is shown.