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. 2016 Mar 14;231(12):2664–2672. doi: 10.1002/jcp.25368

Figure 6.

Figure 6

Overexpression of AR protein in PC‐3 cells decreases PPARγ function. (A) PC‐3 cells were transfected with either the PCR3.1 or PCR‐AR expression vector. Western blot analysis was then used to measure the level of AR, PPARγ, and actin protein in transfected cells. (B) PC‐3 cells were transfected with the PPRE3‐ luciferase reporter plasmid, CMV β‐galactosidase plasmid, and either PCR‐AR or PCR3.1 expression vector. The transfected cells were next treated with DMSO vehicle or different concentrations of rosiglitazone (10–40 μM) for 24 h. Luciferase activity in treated cells was then measured and normalized to β‐galactosidase activity. (C) PC‐3 cells transfected with either the PCR3.1 or PCR‐AR expression vector were treated for 24 h with DMSO vehicle (−) or 40 μM rosiglitazone (+). qRT‐PCR was used to measure the level of FABP mRNA and 18S rRNA in treated cells. In parts B and C, each bar represents the mean ±SEM of three wells. *P < 0.05 compared to the PCR3.1, DMSO group. **P < 0.05 compared to the PCR‐AR, DMSO group.