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. 2016 Nov 23;31(11):1888–1894. doi: 10.1111/jgh.13388

Figure 1.

Figure 1

Monocyte levels in tissues and circulation in experimental hepatopulmonary syndrome. The lung, liver, heart, kidney, brain, and spleen tissues as well as circulating peripheral blood mononuclear cell were obtained from sham, 1 week, and 3 weeks common bile duct ligation (CBDL) animals. Monocyte accumulation in tissues were assessed by the protein levels of ED1 (CD68, a pan monocyte marker) using western blots or immunohistochemistry. Peripheral monocyte levels were assessed by flow cytometry (CD68+/CD172a+, shown by red square). ED1 protein levels were normalized to the levels of glyceraldehyde phosphate dehydrogenase (GAPDH). Increased monocyte infiltration was seen in the lung (a) and liver (b) after CBDL, associated with elevated peripheral monocyte numbers (c). In contrast, splenic monocytes levels decreased after CBDL (d). The levels of monocytes remained unchanged in the heart (e), kidney (f), and brain (g). Values are expressed as means ± SME (n = 6–8 animals for each group). * P < 0.05 compared with control.