Figure 2.

The effects of spleen removal on pulmonary and hepatic monocyte accumulation. The lung and liver were obtained from common bile duct ligation (CBDL) animals with and without splenectomy. Monocyte levels were assessed by the protein levels of ED1 (CD68, a pan monocyte marker) using western blots or immunohistochemistry. (a) Representative images of immunohistochemical staining for CD68 in the lung and liver; (b) representative immunoblots; and (c) graphical summaries of ED1 protein levels in the lung and liver. Splenectomy significantly decreased monocyte levels in the lung but further increased liver monocyte infiltration. Values are expressed as means ± SE (n = 6–8 animals for each group). ^* P < 0.05 compared with sham. ^‡ P < 0.05 compared with CBDL.