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ROCK inhibition increases cell attachment. (A) Cells were stained with a Calcein AM dye to detect adherent and living cells. Fluorescent images at the 6-hour time-point are shown for all three treatments. Scale bar, 200 μm. (B) Quantification of adherent cells at time-points indicated. *P ≤ 0.05, **P ≤ 0.01 compared with control at that time-point. Error bars represent ± SEM (n ≥ 6). Y-27632, panROCK inhibitor (10 μM); ROCKIV, ROCK2 inhibitor (10 μM).
