Figure 3.

ROCK2 inhibition decreases cofilin and MLC phosphorylation. hESC-RPE cells were treated at the time of plating with Y-27632 or ROCKIV and protein was collected 2 hours later. (A) Total protein lysates were probed with anti–phospho-cofilin, cofilin, and β-actin antibodies. (B) Quantification of phosphorylated cofilin protein over β-actin. (C) Total protein lysates were probed with anti–phospho-MLC, MLC, and β-actin antibodies. (D) Quantification of phosphorylated MLC protein over β-actin. *P ≤ 0.05 compared with control. Error bars represent ± SEM (n ≥ 4).