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ROCK inhibition enhances wound closure in vitro. (A) hESC-RPE cells were scratched following 30 days in culture and treated with either Y-27632 or ROCKIV and wound closure was monitored over an additional 30 days. Control and ROCKIV-treated cells failed to completely close the wounded area. Scale bar, 200 μm (B) Quantification of wound closure at each time-point imaged. *P ≤ 0.05, **P < 0.01 compared with control at that time-point. Error bars represent ± SEM (n = 3).
