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a) General reaction showing the application of IEDDA reactions for protein labeling with a site‐specifically installed S‐allyl handle. b–d) Structures of S‐allyl Cys, tetrazine cores, and fluorophores used in this study. e) Rate constants for the reaction of 2–5 with the model amino acid S‐allyl Cys 1 were measured following the consumption of tetrazines by UV/Vis (f) or fluorescence (g).
