Table 2. Details of primer pairs used for real-time PCR assays.

	5’-3’ primer sequence
Gene name	Forward	Reverse	Amplicon size (bp)	Annealing temperature (°C)	Primer efficiency	GenBank accession no.	Primer reference
aqp-1a	CTACCTTCCAGCTGGTCCTG	TGATACCGCAGCCTGTGTAG	141	62	2.05	BT046625	[16]
aqp-1b	CTGTGGGTCTGGGACATCTT	TAAGGGCTGCTGCTACACCT	153	62	2.00	NM_001140000.1	[16]
aqp-8ab	GGAGCTGCCATGTCAAAGAT	CGCCCCTAGCAATACTACCA	159	60	2.00	KC626879.1	[6]
aqp-10	GGTGTTGGTGATCGGAGTCT	CGCCCTAAACACCTCATCC	121	60	1.68	DW569041	[16]
nka α-1c	GAGAGGGAGACGTACTACTAGAAAGCA	CAGCAAGACAACCATGCAAGA	69	60	2.01	NM_001124459	[26]
nka α-1b	CTGCTACATCTCAACCAACAACATT	CACCATCACAGTGTTCATTGGAT	81	60	2.00	NM_001124460	[26]
fxyd-12a	ATCCCTCCGTCATTGGTCAA	TGCTTCATAACTGCTTTCCCTG	96	62	2.01	NM_001123730.1
fxyd-12b	AGGGCAGCTGACTACAGAGA	TGCATCAGGGTCGTACTCAG	139	55	1.96	BK006250
fxyd-12c	GACCTTCGTTGCAGTCATCA	GGCTTCTCTTCAACCTTTCTTTC	102	62	2.00	BK006251
nkcc-1a	GATGATCTGCGGCCATGTTC	CTCGTTCTTCATCAGCCAGC	100	60	1.97	NM_001123683.1
nkcc-2	CCGCGTGCCCAACATC	GCACGGTTACCGCTCACACT	57	60	2.03	NM_001141520	[18]
clc-3	CGAGGGCATCTACGAATCACA	CTCCTTGGCGTCGAGGAA	61	60	1.95	NM _001173586	[18]
cftr-II	AGAATGGGACCGAGAAGTGG	CTCCCCAAAGTACAGCAGGA	115	62	1.91	NM_001123534.1
guanylin	ATCTGGTCTCTGAAGCCCAC	CCGTTTACACTGCTGCTCTC	115	60	2.02	BT047912.2
claudin-15	GGCACGTCTGAGAAACAACA	TAGGAAGTGGCAGCCTGACT	92	60	2.02	BK006395	[19]
claudin-25b	CCTGTAAGAGGGGTCCATCA	TGACACATGTTCTGCCCTGT	101	60	1.98	BK006399	[19]
occludin	GACAGTGAGTTCCCCACCAT	ATCTCTCCCTGCAGGTCCTT	101	60	2.12	NM_001173656.1	[22]
tricellulin	GGATGCCATGATGGGTAAAC	AGGAAGGCTGGGTCACTCTT	111	60	2.03	DW548339	[22]
jam-1a	TCGGCAGGCTATTCAAGAGT	AGTAGGTTCCTCTGGCACTG	110	60	2.00	GBRB01043957.1
jam-1b	CGTTGCGGAAGGGCGTAG	CCAGCGATGTGTCCGATTTC	146	60	2.00	GBRB01043958.1
zo-1	CAAAGCCAGTGTATGCCCAG	CAGCTTCATACTCGGCCTGA	119	60	1.93	>gnl|SRA|SRR403078.506834.2
cingulin	AAAAGCACCCTGGAGAGACA	TTTGTTCCTCTCCTCCACCC	147	60	2.02	JT824570
e-cadherin	ACTATGACGAGGAGGGAGGT	TGGAGCGATGTCATTACGGA	107	60	1.98	BT058864.1
β-catenin	ATGAGGACGCAGATGACCAA	CTGGGGTGTCTGGGAACTTT	126	62	2.07	NM_001140567.1
gapdh	AAGTGAAGCAGGAGGGTGGAA	CAGCCTCACCCCATTTGATG	96	60	1.85	BT050045	[27]
rnapoii	CCAATACATGACCAAATATGAAAGG	ATGATGATGGGGATCTTCCTGC	157	60	1.80	BG936649	[27]
hprt1	CCGCCTCAAGAGCTACTGTAAT	GTCTGGAACCTCAAACCCTATG	255	60	1.99	BT043501	[27]

Abbreviations: aqp, aquaporin; nka, Na⁺, K^– –ATPase; nkcc, Na⁺, K^–, 2Cl^- co-transporter; clc, chloride channel; cftr-II, cystic fibrosis transmembrane conductance regulator Cl^– channel II; jam, junctional adhesion molecules; zo, zonula occludens; gapdh, glyceraldehyde-3-phosphate dehydrogenase; rnapoii, RNA polymerase 2; hprt1, hypoxanthine phosphoribosyltransferase 1; fxyd-12, fxyd domain-containing ion transport regulator 12.