Table 1. Demographic and clinical data of participants.

	Training Sample			Validation Sample			Follow-up Validation Sample
	ALS	HC	p-values	ALS	HC	p-values	ALS	HC	p-values
n	60	50		21	16		21	15
Male sex, n	39	24	p = .1	10	6	p = .74	12	9	p = .86
Age, y(mean/SD)	59.9(10.88)	60.6 (8.8)	p = .68	62.5 (10.5)	60.6 (9.4)	p = .56	62.9 (10.4)	61.6 (9.2)	p = .69
Handedness(right/left)	52/ 8	47/ 3	p = .33	19 /2	14/ 2	p = .77	19/2	13/2	p = .72
Disease Duration months (mean/SD)*	26.1(19.5)			20.6 (15.9)			25.8 (15.6)
Site of onset(bulbar/spinal/ respiratory)	19/ 40/1			6/15/0			6/15/0
ALSFRS-R(mean, SD)	38.2 (6.2)			39 (7)			35.3(8.2)
Interval between scans months (mean/ SD)							5.3 (1.5)	4.2 (0.8)	p< .05

* Disease duration from symptom onset until date of scan