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RNA was isolated from the wild type (Wt) Xoo strain grown in either PS or XOM2 medium and converted into cDNA which was further subjected to Real Time-PCR analysis. The expression of the pglA, pel, pelL and pmt genes was examined in XOM2 medium using PS as the reference sample and 16SrRNA as an endogenous control. The average fold change (2-∆∆Ct) of three independent experiments was plotted for each of the four genes.
