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. 2016 Dec 1;11(12):e0166396. doi: 10.1371/journal.pone.0166396

Table 1. List of bacterial strains and plasmids used in this study.

Bacterial Strains/Plasmids Relevant characteristic(s) Reference/ source
E. coli strains
DH5α λ f80dlacZDM15 D(lacZYA-argF)U169 recA1 endA hsdR17 (rK mK) supE44 thi-1 gyrA relA1 Invitrogen
X. oryzae pv. oryzae strains
BXO1 Wild type; Indian isolate laboratory collection
BXO43 rif-2; derivative of BXO1 laboratory collection
pglA- pglA-:: pK18mob rif-2; Kmr; pglA-; Kmr This study
pmt- Pmt-:: pK18mob rif-2; Kmr; pmt-; Kmr This study
pel- pel-:: pK18mob rif-2; Kmr; pel-; Kmr This study
pelL- pelL-:: pK18mob rif-2; Kmr; pelL-; Kmr This study
pglA -/pHM1 pglA-/pHM1; rif-2; Kmr, Spr; derivative of pglA- This study
pglA- /pHM1:: pglA pglA-/pHM1:: pglA; rif-2; Kmr, Spr; derivative of pglA- This study
pmt-/pHM1 pmt- /pHM1; rif-2; Kmr, Spr; derivative of pmt- This study
pmt- /pHM1:: pmt pmt- /pHM1:: pmt; rif-2; Kmr, Spr; derivative of pmt- This study
Plasmids
pK18mob pUC18 derivative; Mob+ Tra- Kmr; does not replicate in X. oryzae pv. oryzae [19]
pTL1 pK18mob+759 bp of internal fragment of pglA gene This study
pTL2 pK18mob+793 bp of internal fragment of pmt gene This study
pTL3 pK18mob+767 bp of internal fragment of pel gene This study
pTL4 pK18mob+653 bp of internal fragment of pelL gene This study
pHM1 Broad-host-range cosmid vector (13.3kb); Spr [20]
pTC5 pHM1+ 1853 bp gene fragment containing 1671 bp pglA gene This study
pTC6 pHM1+ 1252 bp gene fragment containing 1224 bp pmt gene This study