E. coli strains |
|
|
DH5α |
λ– f80dlacZDM15 D(lacZYA-argF)U169 recA1 endA hsdR17 (rK– mK–) supE44 thi-1 gyrA relA1
|
Invitrogen |
X. oryzae pv. oryzae strains |
|
|
BXO1 |
Wild type; Indian isolate |
laboratory collection |
BXO43 |
rif-2; derivative of BXO1 |
laboratory collection |
pglA-
|
pglA-:: pK18mob rif-2; Kmr; pglA-; Kmr
|
This study |
pmt-
|
Pmt-:: pK18mob rif-2; Kmr; pmt-; Kmr
|
This study |
pel-
|
pel-:: pK18mob rif-2; Kmr; pel-; Kmr
|
This study |
pelL-
|
pelL-:: pK18mob rif-2; Kmr; pelL-; Kmr
|
This study |
pglA
-/pHM1 |
pglA-/pHM1; rif-2; Kmr, Spr; derivative of pglA-
|
This study |
pglA- /pHM1:: pglA
|
pglA-/pHM1:: pglA; rif-2; Kmr, Spr; derivative of pglA-
|
This study |
pmt-/pHM1 |
pmt- /pHM1; rif-2; Kmr, Spr; derivative of pmt-
|
This study |
pmt- /pHM1:: pmt
|
pmt- /pHM1:: pmt; rif-2; Kmr, Spr; derivative of pmt-
|
This study |
Plasmids |
|
|
pK18mob
|
pUC18 derivative; Mob+ Tra- Kmr; does not replicate in X. oryzae pv. oryzae
|
[19] |
pTL1 |
pK18mob+759 bp of internal fragment of pglA gene |
This study |
pTL2 |
pK18mob+793 bp of internal fragment of pmt gene |
This study |
pTL3 |
pK18mob+767 bp of internal fragment of pel gene |
This study |
pTL4 |
pK18mob+653 bp of internal fragment of pelL gene |
This study |
pHM1 |
Broad-host-range cosmid vector (13.3kb); Spr
|
[20] |
pTC5 |
pHM1+ 1853 bp gene fragment containing 1671 bp pglA gene |
This study |
pTC6 |
pHM1+ 1252 bp gene fragment containing 1224 bp pmt gene |
This study |