Fig 1. fMYFINILTL and MYFINILTL induced superoxide production from human neutrophils.
Neutrophil NADPH-oxidase activity was measured by an isoluminol/HPR chemiluminescence system. (A) Neutrophils were pre-incubated at 37°C for 5 min in the absence (solid line) or presence of FPR1 antagonist Cyclosporin H (CysH, 1 μM, dashed line) or FPR2 antagonist PBP10 (1 μM, dotted line) before stimulation with fMYFINILTL (10 nM; time for addition indicated by an arrow). The curves are from one representative response (n > 10). Inset: The superoxide production induced by the non-formylated peptide variant MYFINILTL (1 μM) in the presence or absence (control) of FPR specific inhibitors. Abscissa, Time of study (min); ordinate, superoxide production (in arbitrary light units, cpmx10-6). (B) Neutrophil NADPH-oxidase activity induced by different concentrations of fMYFINILTL. The peak values of the responses in relation to the concentration of fMYFINILTL was determined and is expressed as a percent of the maximal response. Data are expressed as mean ± SD from four independent experiments. Abscissa, agonist concentration (M); ordinate, superoxide production (percent of max).