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. 2016 Dec 1;11(12):e0166735. doi: 10.1371/journal.pone.0166735

Fig 5. Ellipsometric Imaging.

Fig 5

a: Time-series of contrast ellipsometric images of lipids (82% DOPC, 15%DOPS, 3% NBD-PE) spreading from a source on Silicon with a 100nm of oxide in PBS solution, without a fluorescence filter-cube in the beampath. Images have been corrected for oblique incidence geometric distortion, and the contrast globally enhanced for visibility. b: Fluorescence microscopy image of the same location without moving the sample, by inserting the fluorescence filter-cube and disabling the ellipsometry laser. c: Null-angle as determined by fitting the raw intensity vs. polarizer angle to a parabola. d: Null polarizer angle as a function of position across the profile noted in e. e: Null map of the edge of the a lipid bilayer with the same composition, on bare Silicon in PBS buffer.