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. 2016 Dec 1;11(12):e0167599. doi: 10.1371/journal.pone.0167599

Fig 1. Cytogenetic analysis of U-2946 cells.

Fig 1

A) Classical and B) Spectral Karyotyping shows key oncogenomic rearrangements present in U-2946 cells. BAC clones used for FISH are shown along with corresponding genomic copy number plots. Blue arrows show t(8;14)(q24;q32), red arrows der(8)t(8;8)(p22;q24), yellow arrows der(9)t(2;9) and purple arrows add(17)(p11). Note differences between cells in A and B reflecting clonal heterogeneity indicated by stars. C-G) Image depicts analysis of t(8;14) by chromosome painting (C), and single locus FISH using probes for IGH (D) and MYC (E). Positions of BAC clones are shown for IGH (F) and MYC loci (G). Note partial duplication of the terminal der(14) long arm region including IGH/MYC (arrow) present in a subclone (C). H-M) Analysis of MCL1/MAP2K3 coamplification: gold signals from MCL1 clone (RP11-54A4), barely visible at native loci (arrows), are intense on der(17) due to amplification (H). Similarly, green signals from 17p11 clone (RP11-64J19) covering MAP2K3 are amplified, while red signals (RP11-160E2) from the neigboring hemizygously deleted region are present on the unrearranged chromosome only. Chromosome painting shows localization of amplicon to 17p (K). Genomic copy number plots show consistency with FISH amplification data for both MCL1 (L) and MAP2K3 (M). N-O) Cytoscan HD array analysis revealed prominent amplification of 1q21.3 (N) and 17p11.2 (O).