Fig 6. Inhibition of BCL2 and MCL1: effect on apoptosis.

Apoptosis was assessed by Annexin-V staining, 24 h after addition of a BCL2 inhibitor (ABT-263) or an MCL1 inhibitor (A-1210477). The MCL1 inhibitor triggered apoptosis in the MCL1^pos/BCL2^neg cell line U-2946, BCL2 inhibition induced apoptosis in the MCL1^pos/BCL2^pos cell line U-2932. Although expressing MCL1, cell line U-2932 was not responsive to MCL1 inhibition, suggesting dominant function of BCL2. The data were reproduced in two independent experiments.