Fig 6. Knockdown of Rpd3 reduces the level of Atg9 mRNA that is induced in response to starvation.

Relative Atg9 mRNA levels were measured by RT-qPCR with Canton S (WT) or Rpd3 knockdown (w; Fb-GAL4 /+; HDAC1^JF01401/+) adult male flies at indicated hours starved. The Atg9 mRNA levels transiently increased at 3 h and 6 h starvation in wild type flies, but not in the Rpd3 knockdown flies. Since Rpd3 knockdown flies die at 36 h and 48 h starvation, data at these time points were not obtained with these flies. *P-value < 0.05 and ** P-value < 0.01, n = 3.