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. 2016 Dec 1;11(12):e0167627. doi: 10.1371/journal.pone.0167627

Fig 3. UHPLC-ESI-QTOF-MS analysis of the main metabolites in the culture medium of L. theobromae strain 2334.

Fig 3

L. theobromae strain 2334 was cultivated in medium supplemented with KNO3 for 9 d. After extraction, compounds of the culture filtrate were analyzed via UHPLC-ESI-QTOF-MS. Shown is a total ion chromatogram (A), as well as high resolution MS/MS-spectra (negative ionization mode; collision energy 10 eV) of different compounds (B-H). Proposed structures and fragmentations are shown as inset for each spectrum. The following compounds were identified: dihydroxy-dihydro-JA ([M-H]- 243.1212, RT 3.40 min) (B), 8-hydroxy-JA ([M-H]- 225.1120, RT 3.78 min) (C), hydroxy-dihydro-JA ([M-H]- 227.1280, RT 4.29 min) (D), Indol-3-carboxylic acid ([M-H]- 160.0390, RT 4.54 min) (E), JA ([M-H]- 209.1178, RT 5.56 min) (F), 9,10-dihydro-JA ([M-H]- 211.1334, RT 5.88 min) (G), Isoleucyl-C11H16O2. ([M-H]- 310.2028, RT 6.37 min) (H). Abbreviations: JA, jasmonic acid.