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. 2016 Nov 29;107(11):1705–1716. doi: 10.1111/cas.13075

Figure 5.

Figure 5

Effect of JAG1 gene silencing on proliferation and epithelial mesenchymal transition (EMT)‐like phenotype based on in vitro study. (a, b) Effect of small interfering RNA for JAG1 (siJAG1) on JAG1 expression and EMT‐like phenotype. E‐cadherin and SNAIL protein expression (a) or E‐cadherin (CDH1) and vimentin (VIM) mRNA expression (b) were analyzed by western blotting and qRT‐PCR respectively. Non‐targeting siRNA (siNON) was used as a negative control. Expression levels of mRNA are indicated relative to expression with siNON treatment. Data are mean ± SD of nine wells. (c) Effect of siJAG1 on JAG1 and HES1 mRNA expression in p53 −/− and wild type (Wt) HCT‐116 cells. mRNA expression was presented as a ratio relative to expression in Wt cells treated with siNON in the left two panels. JAG1 mRNA expression in Wt or p53 −/− cells treated with siJAG1 was also presented as a ratio relative to expression in Wt or p53‐/‐ cells treated with siNON in the right panels. Data are mean ± SD of nine wells. (d) Effect of siJAG1 on JAG1 protein expression in p53 −/− and Wt cells analyzed by western blotting. (e) Effect of siJAG1 on cell growth in p53 −/− and Wt cells. siJAG1 treatment was initiated 2 days after plating. Data are presented as mean ± SD of six wells for each time point (result from three independent experiments). Statistical analysis was performed by Student's t‐test. * or #, P < 0.05.