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. 2016 Nov 29;107(11):1632–1641. doi: 10.1111/cas.13074

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Analysis of the effect of cellular SULF‐2 levels on Wnt, VEGF or FGF regulation of cell invasion. Cells invading the BD Matrigel Basement Membrane Matrix are shown (a). The effect of cellular SULF‐2 level on Wnt (b), VEGF (c) or basic FGF (d) regulation of cell invasion was determined using a cell invasion assay. Fold change in cell invasion compared to non‐treated control is shown. For (C), *P < 0.001, **P = 0.01 (compared with NC siRNA‐transfected cells with VEGF stimulation), ***P = 0.001 (compared with SULF‐2 plasmid‐transfected cells with VEGF stimulation) and for (d), *P = 0.02, **P = 0.008 (compared with NC siRNA‐transfected cells with FGF stimulation), ***P < 0.001(compared with SULF‐2 plasmid‐transfected cells with FGF stimulation).