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. 2016 Dec 1;11(12):e0167555. doi: 10.1371/journal.pone.0167555

Fig 5. Cultured TSC lines support ILC survival in vitro.

Fig 5

(A) FACS sorted ILCs were co-cultured with either TSC (harvested at passage 2 or 3), 50ng/mL rhIL-7 or medium alone for 4 days. Shown are representative plots of FSC-A vs SSC-A profile as well as staining for CD45 and fixable viability marker to assess ILC viability upon culture. Plots are representative for 4 independent experiments. (B) Percentages of viable ILCs upon co-culture with either TSC line, rhIL-7 or medium alone for 4 days. n = 5 individual donors. (C) FACS sorted ILCs were co-cultured with TSC harvested at passage 4, TSC harvested at passage 14 or without stroma for 4 days. Shown are percentages of viable ILCs at day 4. n = 3 individual donors. For all plots: *: p<0.05, ***: p<0.001, Two way ANOVA with Bonferroni correction. Error bars represent mean ± SD.