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. 2016 Dec 1;11(12):e0167550. doi: 10.1371/journal.pone.0167550

Fig 2. Phase-contrast images of bovine-induced trophoblastic cells (biTBCs).

Fig 2

(A) Bovine amniotic cells (bADCs). (B) iPSC-like colonies appearing in the culture medium used for human iPSCs. (C) Trophoblast-like colonies appearing in the culture medium used for human iPSCs. (D) Primary trophoblast-like colonies appearing in the trophoblast cell medium at day 14. (E) Established biTBCs derived from bADCs. (F) Established biTBCs derived from bovine fetal liver cells (bFLCs). (G) Formation of trophoblastic vesicles (TVs) in central areas of colonies during culture. (H) Established biTBCs cultured on collagen-coated dishes. (A)-(H) scale bars = 500 μm.