Skip to main content
. 2016 Dec 1;11(12):e0166135. doi: 10.1371/journal.pone.0166135

Fig 2. Flow cytometry signals with day 70 serum on MOA bulk and MOA clones are variable.

Fig 2

(A) Mean fluorescence intensities (MFI) obtained with MOA serum of day 70 followed by staining with a secondary antibody attached to FITC shows variable surface recognition signals ranging from low (< 80 MFI) to medium (81–160 MFI) and high (> 160 MFI) in MOA bulk and in the MOA clones. Error bars reflect the mean error of at least three independent experiments (Student´s t-test: MOA bulk vs. D5: p = 0.008, A1 vs. H6: p = 0.0003, D2 vs. D5: p = 0.0001, D2 vs. H6: p = 0.008, D5 vs. A1: p = 0.0002). (B) and (C) show transmission and scanning electron microscopy graphs of two representative clones with a highly significant difference in surface recognition signal (MOA D2 and MOA D5) and the presence of knobs (arrows) in equal numbers. Knobs were quantified per TEM cut of each clone.