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. 2016 Dec 1;11(12):e0167420. doi: 10.1371/journal.pone.0167420

Fig 1. SP induces HO-1 mRNA and protein expression.

Fig 1

RAW 264.7 cells were treated with the indicated concentrations of SP for (A) 4, (B) 6 and (C) 24 hours and mRNA expression levels were assessed by RT-qPCR. (B). The results shown are the means ± SD of two experiments (each of which was performed in triplicate). *p<0.05 and **p<0.01 versus each agent alone. (D) Cells were treated with SP (μM) for 24 hours. The induction of HO-1 protein expression (32 kDa) was analysed by western blotting. The numbers represent the fold of HO-1 difference with untreated control samples (Ctrl) arbitrarily set at 1.0. The data represent two independent representative experiments.