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. 2016 Dec 1;11(12):e0167420. doi: 10.1371/journal.pone.0167420

Fig 6. HO-1 induction promotes M2 macrophage polarisation.

Fig 6

RAW 264.7 cells were transfected with siRNA HO-1 and siRNA NC (non-correlated). (A) RT-qPCR analysis of HO-1 mRNA in cells transfected 48 hours after transfection. Cells were treated with SP (10 μM) and/or LPS (100 ng/ml) for 24 hours and mRNA expression of selected genes was evaluated by RT-qPCR (B) IL-6 mRNA expression (C) TNF-α mRNA expression (D) Arg1 mRNA expression (E) IL-10 mRNA expression and (F) Rel A mRNA expression. The data are mean ±SD of two separate experiments, each of which was performed in triplicate. **p < 0.01 versus siRNA NC.