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. 2016 Dec 1;5:e20621. doi: 10.7554/eLife.20621

Figure 1. Alignment and structure of the conserved apicomplexan protein CelTOS from the human pathogen Plasmodium vivax (PvCelTOS).

(A) Alignment of CelTOS from apicomplexan parasites Plasmodium, Babesia, Theileria, Cytauxzoon, and mapping of the structural elements. In the alignment, grey shading represents similarity, black shading represents identity. The secondary structure features are based on the crystal structure of PvCelTOS and shown in green. Protein accession codes as follows: Plasmodium vivax [UniProtKB - A5JZX5], Plasmodium falciparum [UniProtKB - Q8I5P1], Babesia microti [UniProtKB - I7J9D8], Theileria parva [UniProtKB - Q4N982], Cytauxzoon felis [PiroplasmaDB - CF003135]. (B) PvCelTOS is an alpha helical dimer that resembles a tuning fork. Each monomer is in green or white ribbon and surface representation. (C) Surface maps of CelTOS dimer showing the electrostatic surface potential colored from red (−5 kT e−1) to blue (5 kT e−1). (D) The CelTOS monomer shown as ribbon representation can be separated into two distinct subdomains composed of N-terminal (α-helices 1 and 2) and C-terminal helices (α-helices 3 and 4). (E) Surface maps of CelTOS monomer reveals the inner hydrophobic surfaces composed of the dimer interface and one face of the tuning fork prongs. Coloring as in Figure 1C.

DOI: http://dx.doi.org/10.7554/eLife.20621.003

Figure 1—source data 1. Data collection, phasing and refinement statistics.
DOI: 10.7554/eLife.20621.004
Figure 1—source data 2. Sedimentation equilibrium analytical ultracentrifugation analysis for Pf and PvCelTOS.
Three independent global fits for three concentrations and two speeds demonstrate Pf and PvCelTOS are dimers in solution. The theoretical monomer molecular weight for PfCelTOS and PvCelTOS are 18.655 kDa and 18.665 kDa, respectively.
DOI: 10.7554/eLife.20621.005

Figure 1.

Figure 1—figure supplement 1. Alignment of CelTOS from Apicomplexan parasites and mapping of the structural elements.

Figure 1—figure supplement 1.

In the alignment, grey shading represents similarity, black shading represents identity. Protein accession codes for CelTOS from the following Apicomplexan parasites are as follows: Plasmodium falciparum [UniProtKB - Q8I5P1], Plasmodium vivax [UniProtKB - A5JZX5], Plasmodium berghei [UniProtKB - Q4YC08], Plasmodium knowlesi [UniProtKB - B3LCG1], Plasmodium reichenowi [UniProtKB - A0A060RVP1], Plasmodium yoelii [UniProtKB - Q6T944], Plasmodium chabaudi chabaudi [UniProtKB - A0A077TR60], Plasmodium fragile [UniProtKB - A0A0D9QNC3], Plasmodium inui [UniProtKB - W7ABI3], Plasmodium vinckei vinckei [UniProtKB - W7B0C7], Plasmodium vinckei petteri [UniProtKB - W7B0C7], Babesia microti [UniProtKB - I7J9D8], Babesia bovis [UniProtKB - A7AQQ4], Babesia bigemina [UniProtKB - A0A061DAC2], Babesia equi [UniProtKB - L0AZQ6], Theileria parva [UniProtKB - Q4N982], Theileria annulata [UniProtKB - Q4UGH3], Theileria orientalis [UniProtKB - J7M8E0], Theileria equi [NCBI Reference Sequence: XP_004830035.1] and Cytauxzoon felis [PiroplasmaDB - CF003135].
Figure 1—figure supplement 2. Electron-density maps.

Figure 1—figure supplement 2.

2Fo-Fc electron density contoured at 1.5σ around a representative section of PvCelTOS.