Figure 6. Induction, maintenance, and chaperone dependence of a lactic acid-induced [GAR+]-like state is conserved in the evolutionary distant yeast Dekkera bruxellensis.
(A) Induction of a [GAR+]-like state in D. bruxellensis. [gar−] cells were plated on GLY + GlcN (containing 0.1–0.15% glucosamine) medium with or without 0.02% D-lactic acid. Control cells harbor spontaneous [GAR+]. For each of the treatment images, four biological replicates are shown, in five-fold serial dilutions. (B) Maintenance and chaperone dependence of the lactic acid-induced [GAR+]-like state in D. bruxellensis. Cells induced in A. were propagated non-selectively for ~75 generations by restreaking on YPD plates, then subsequently retested for maintenance of the prion phenotype on GLY + GlcN (0.1–0.15% glucosamine), with or without the Hsp70 inhibitor myricetin (50 μM). Four biological replicates are shown for each induced group, and two shown for the uninduced and propagated [gar−] cells.