Figure 7. m18 is dispensable for MCMV growth in vitro and acutely in vivo.

(A) MCMV titers in supernatents of NIH-3T3 cells infected with either WT or ∆18 MCMV. (B) qPCR analysis of MCMV genome copy number from spleen, lung, and liver tissues of BALB/c mice infected with 500,000 p.f.u. of WT or MCMV^m18stop. (C) qPCR analysis of MCMV genome copy number from spleen, lung, and liver tissues of RAG^-/-γC^-/- Mice infected with 50,000 p.f.u. of WT or MCMV^m18stop.

DOI: http://dx.doi.org/10.7554/eLife.14749.013

Figure 7—figure supplement 1. MCMV^m18stop does not induce RAE-1 expression or cause a defect in IE-1 expression.

(A) RT-qPCR analysis of Raet1 expression in mouse fibroblasts infected with WTMCMV or MCMV^m18stop. (B) RT-qPCR analysis of MCMV IE-1 expression in mouse fibroblasts infected with WTMCMV or MCMV^m18stop. **p<0.05. n.s., Not significant (Students T-test).

DOI: http://dx.doi.org/10.7554/eLife.14749.014