Table 2.

BAC mutagenesis primers.

DOI: http://dx.doi.org/10.7554/eLife.14749.018

Primer pair	Forward	Reverse
Kanamycin*	CGATTTATTCAACAAAGCCACGTTGTGTCT	GCCAGTGTTACAACCAATTAACCAATTCTGA
Δ1-6	gtgtcacgcgcacgtgttagcataggaatccagacgcgcgctcgcctgag	atttacatactcaggacaggtgtgggcggttccaggtgtacgtaagcaga
Δ6-12	acacgcccaaaatcacgcaatcatatataaatggacaatgaagccaatct	gttctaagtaaaaggggatacgggcgggcgatacagatgtacgaacccaa
Δ12-18	caacaaataaaaattgtacgctcattttatcgcgtctctgtcatgtgttc	gaggggttggtacggttcgagcgattttggtagtccgagacgtccgccgc
Δ18-22	ttgaatacgattgtttttattggcagcactgagcacacgtcccccccacc	atccgctcgaggccatgctcaccaagaagaccgagtgtcccaacaacttc
Δ1-22	gtgtcacgcgcacgtgttagcataggaatccagacgcgcgctcgcctgag	atccgctcgaggccatgctcaccaagaagaccgagtgtcccaacaacttc
Δ12	caacaaataaaaattgtacgctcattttatcgcgtctctgtcatgtgttc	gttctaagtaaaaggggatacgggcgggcgatacagatgtacgaacccaa
Δ13	tagaacaatatgtaaaccatctctcattcagctacatacagacaagggac	Tgataagaattatactttaatgggggacacgttctagaacacgataaact
Δ14	Agtataattcttatcaattataccagagtttggtatttttttaatctgag	Cgagtgtgaaatggggaaactggcgcgtcttttcattcgtgctccacagc
Δ15/16	cactcgctatccttcgaccacactttcgagtcttttaccgtatcaagaag	Acaataaagatttcagacaaaaagtatggattgtgtgataatttattaaa
Δ17	catactttttgtctgaaatctttattgtacgccatcgaaataaggggagc	gtctgctttctttgaaatcggacgaccgatcagaacgtccgccttcgaga
∆18	ttgaatacgattgtttttattggcagcactgagcacacgtcccccccacc	Gaggggttggtacggttcgagcgattttggtagtccgagacgtccgccgc
∆19	ccaagacgctcgtcttataacaccgactgacgtttactccgactcaggat	Tcgaggcgagtcttcggagctgtacgctagggcgatcgccatcaccctct
∆20/21	Cggcgacgacggcgatcacggcgagggtgaagagggtgatggcgatcgcc	Gctgtcatgtaaatggacggttattaaaagatgaggtcgtgtgacctctg
∆22	Gggtagcgcctcgatcgacgagcgtcggacaaagaaaccgggagaagaag	Atccgctcgaggccatgctcaccaagaagaccgagtgtcccaacaacttc
MCMVm18stop	gcagcggttccgccgtccccatcgcgacgatgggcgctccgaattcctaataaaccgactcccgtccccaccaaggatgacgacgataagtaggg	ggagcgcccatcgtcgcgatttattaggaattccgcgctgctggcgatgagcgtggtggggacgggagtcggtaaccaattaaccaattctgattag

*Kanamycin specific oligos were added to the 3 the 3ggaattccgcgctgctggcgatgagcgtggtgggga.