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. Author manuscript; available in PMC: 2017 Nov 23.
Published in final edited form as: Cell Syst. 2016 Nov 23;3(5):434–443.e8. doi: 10.1016/j.cels.2016.10.020

Figure 1. A multi-step process was used to reconcile a few existing unpublished models and to generate the final community reconstruction and models.

Figure 1

(A) The manually-curated human metabolic network reconstructions (Recon 1 (Duarte et al., 2007) and Recon 2 (Quek et al., 2014)) were used to define an initial set of reactions catalyzed in C. griseus and the genes and proteins involved in each reaction (GPRs). Specifically, Recon 1 and Recon 2 were combined and all enzyme-catalyzed reactions that differed between the two were manually curated and reconciled to obtain consistent GPRs. C. griseus homologs were obtained for each human gene to obtain a set of draft GPRs linked specifically to genes in the CHO-K1 genome annotation. (B) The draft CHO GPRs were then compared with the GPRs from three independently-reconstructed and unpublished CHO genome-scale models, thus leveraging the manual curation invested in each input model. By manually verifying all GPRs and adding additional CHO-specific reactions present in the input CHO genome-scale models, we obtained a more comprehensive community reconstruction for C. griseus. To enable computation with this network, orphan reactions from Recon 2 were added, and omics data were used to build a global and cell-line specific models.