Table 1.
Sequence names, accession numbers, and primer sequences for genes examined for hepatic transcriptional levels.
| Gene | Main Function | Accession Number | Forward Primer | Reverse Primer |
|---|---|---|---|---|
| Fatty acid synthase (Fasn) | Fatty acid synthesis | NM_017332 | CCATCATCCCCTTGATGAAGA | GTTGATGTCGATGCCTGTGAG |
| Stearoyl-CoA 9-desaturase; Stearoyl-CoA desaturase 1 (Scd1) | Fatty acid desaturation | NM_139192 | TCAATCTCGGGAGAACATCC | CATGCAGTCGATGAAGAACG |
| Diacylglycerol O-acyltransferase 1 (Dgat1) | Triglyceride synthesis | NM_053437 | AATGCTGCGGAAAAACTACG | TTGCTGGTAACAGTGCTTGC |
| Diacylglycerol O-acyltransferase 2 (Dgat2) | Triglyceride synthesis | NM_001012345 | AATCTGTGGTGCCGCCAG | TCCCTGCAGACACAGCTTTG |
| Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam) | Triglyceride synthesis | NM_017274 | AATGCTGCGGAAAAACTACG | TTGCTGGTAACAGTGCTTGC |
| Sterol regulatory element-binding protein 1c (Srebf1) | Key regulator of fatty acid/triglyceride synthesis | NM_001271207 | GAACCGCAAAGGCTTTGTAAA | ACCCAGATCAGCTCCATGGC |
| Hydroxymethylglutaryl-CoA synthase, cytoplasmic (Hmgcs1) | Sterol synthesis | NM_017268 | CAGCTCTTGGGATGGACGA | GGCGTTTCCTGAGGCATATATAG |
| Sterol regulatory element-binding protein 2 (Srebf2) | Key regulator of sterol synthesis | NM_001033694.1 | GCCGCAACCAGCTTTCAA | CCTGCTGCACCTGTGGTGTA |
| Peroxisome proliferator-activated receptor alpha (Ppara) | Fatty acid oxidation | NM_013196 | AATGCAATCCGTTTTGGAAGA | ACAGGTAAGGATTTCTGCCTTCAG |
| Peroxisome proliferator-activated receptor gamma (Pparg) | Adipocyte differentiation | NM_001145366 | CCACAAAAAGAGTAGAAATAAATGTCAGTAC | CAAACCTGATGGCATTGTGAGA |
| Insulin receptor substrate 2 (Irs2) | Mediation of insulin effects | NM_001168633 | GAAGCGGCTAAGTCTCATGG | CTGGCTGACTTGAAGGAAGG |
| Phosphorylase, glycogen, liver (Pygl) | Glycogen breakdown | NM_022268 | AAAAGCCTGGAACACAATGG | TCGGTCACTGGAGAACTTCC |
| Mechanistic target of rapamycin (Mtor) | Mediation of cellular metabolic stress response | NM_019906 | CATGAGATGTGGCATGAAGG | AAACATGCCTTTGACGTTCC |
| Carbohydrate responsive element binding protein (ChREBP/Mlxipl) | Triglyceride synthesis in response to carbohydrates | AB074517 | CTCTCAGGGAATACACGTCTCC | ATCTTGGTCTTTGGGTCTTCAGG |
| β-actin (Actb) | Endogenous control | NM_031144 | TACAGCTTCACCACCACAGC | CTTCTCCAGGGAGGAAGAGG |
| Rattus norvegicus ribosomal protein lateral stalk subunit P0 (Rplp0) | Endogenous control | NM_022402.2 | CATTGAAATCCTGAGCGATGT | AGATGTTCAACATGTTCAGCAG |