Table 2.
Composition of experimental diets 1–3.
| Ingredient Standard Bean Diet Biofortified Bean Diet | ||
|---|---|---|
| g/kg (by Formulation) | ||
| Biofortified Beans (88 µg Fe/g),MIB465 | − | 400 |
| Standard Beans (59 µg Fe/g), DOR500 | 400 | − |
| Corn | 350 | 350 |
| Corn oil | 30 | 30 |
| Dry skim milk | 100 | 100 |
| Corn starch | 46.75 | 46.75 |
| Vitamin/mineral premix (no Fe) 1 | 70 | 70 |
| dl-Methionine | 2.5 | 2.5 |
| Choline Chloride | 0.75 | 0.75 |
| Total (g) | 1000 | 1000 |
| Selected components | mean ± SEM, n = 5 (by analysis) | |
| Fe (µg Fe/g) 2 | 39.4 ± 0.2 b | 52.9 ± 0.9 a |
| Phytate:Fe molar ratio 3 | 8.25 ± 0. 65 a | 8.95 ± 0.72 a |
1 Vitamin and mineral premix provided/kg diet (330,002 Chick vitamin mixture; 230,000 Salt mix for chick diet; Dyets Inc., Bethlehem, PA, USA); 2 Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co., Franklin, MA, USA) following wet ashing; 3 Method for determining phenol concentrations and phytate contents are described in the materials and methods section; a,b: Within a row, means without a common letter are significantly different, p < 0.05.