Table 1.
Antibodies used for flow cytometry analysis
| Tube | Antibody | Flourochrome | Purpose |
|---|---|---|---|
| 1 | Mouse anti-human CD90 | FITC | Compensation |
| 2 | Mouse anti-human CD44 | PE | Compensation |
| 3 | Mouse anti-human CD105 | PerCP | Compensation |
| 4 | Mouse anti-human CD73 | APC | Compensation |
| 5 | hMSC positive cocktail and hMSC negative cocktail | FITC (CD90), PerCP (CD015), APC (CD73), PE (CD34, CD11B, CD19, CD45, HLA-DR) | Evaluation of positive and negative MSC markers |
| 6 | Unstained | NA | Control |
| 7 | hMSC positive isotype control and PE hMSC negative isotype control | FITC (CD90), PerCP (CD015), APC (CD73), PE (CD34, CD11B, CD19, CD45, HLA-DR) | Negative staining control |