Figure 1.

Isolation of B cells early in development. (A) B cell development pathway with phenotype used to distinguish each cell type. Starting from a CD19⁺ population: (B) Example showing the sorting strategy used to isolate pre-B (red: IgK⁻IgL⁻CD38⁺IgM⁺) and immature (orange: IgK⁺ or IgL⁺CD27⁻IgM⁺IgD⁻CD10⁺) B cells from bone marrow mononuclear cells (BMMCs). (C) Sorting strategy used to isolate transitional (green: IgD⁺CD27⁻CD10⁺) and naïve (blue: IgD⁺CD27⁻CD10⁻) cells from matched peripheral blood mononuclear cells (PBMCs). Dotted lines on the plots represent the gates based on FMO controls, and the solid lined boxes represent the gating used to collect the different subsets.