Figure 6.

Transitional cells have a unique heavy chain immunoglobulin repertoire. (A,C) Minkowski distance clustering analysis of heavy chain VDJ family usage (A) and CDRH3 Kidera factors for pre-B (P) immature (I), transitional (T), and naïve (N) cells in each donor (C). (B) The frequency of gene use (%) for different cell types in each individual donor for genes that have a distinctive distribution in transitional cells. (D) CDRH3 physicochemical properties in different cell types for properties that have distinctive distributions in transitional cells (*p < 0.05 ANOVA). Values on the y axis are as per the individual graph titles. (E) High-dimensional clustering of CD24^hiCD38^hi transitional B cells indicates heterogeneity within the transitional population with respect to IgM expression, illustrated as a SPADE plot. Populations numbered 1–13 have been grouped according to the expression of IgM, IgD, CD21, and CD23, as shown in Figure S1 in Supplementary Material.