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. 2016 Dec 2;7:1951. doi: 10.3389/fmicb.2016.01951

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Copyright © 2016 Navarro-Arias, Defosse, Dementhon, Csonka, Mellado-Mojica, Dias Valério, González-Hernández, Courdavault, Clastre, Hernández, Pérez-García, Singh, Vizler, Gácser, Almeida, Noël, López, Papon and Mora-Montes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorophore-assisted carbohydrate electrophoresis of O-linked mannans. Upon β-elimination, O-linked mannans were derivatized with 8-aminonaphthalene-1,3,6-trisulfonic acid and sodium cyanoborohydride and separated in a 35% (w/v) polyacrylamide gel under non-denaturing conditions. The image is representative of three independent experiments. The strains used are NGY152 (WT) KU141 (Parental), HMY134 (pmr1Δ), and HMY138 (pmr1Δ + PMR1). The molecular marker used was a ladder of maltooligosaccharides from one (G1) to seven (G7) glucose units.