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mRNA and protein expression levels of brain natriuretic peptide (BNP) in the spinal cord. (A) RT-PCR analysis of BNP expression in DRG and spinal cord. −RT, samples without reverse transcription. BNP band appeared at the expected size (321 bp). (B) Western blot analysis was performed using a polyclonal antibody raised against BNP. The antibody recognized a single band at approximately 33 kDa in the spinal cord.
