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. 2016 Dec 2;10:116. doi: 10.3389/fnana.2016.00116

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Copyright © 2016 Abdelalim, Bellier and Tooyama.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence confocal photomicrographs, showing the co-localization of BNP and calcitonin gene-related peptide (CGRP) in rat spinal cord. Spinal cord sections were labeled for BNP (red) and CGRP (green). (A) BNP immunoreactivity in the DH of the spinal cord. (B) CGRP-immunoreactive fibers in laminae I–II. (C) Merged images show the co-localization of BNP and CGRP in superficial laminae I–II. (D) A low-magnification image shows BNP immunoreactivity in the VH. (E) A low-magnification image shows CGRP immunoreactivity in the VH. (F) Merged images the co-localization of BNP and CGRP in the VH. (G) A high-magnification image shows BNP-immunoreactive neurons in the VH. (H) A high-magnification image shows CGRP-immunoreactive motor neurons in the VH. (I) Merged images show the co-localization of BNP and CGRP in the VH motor neurons. Scale bars in (A–C) and (G–I) = 100 μm and in (D–F) = 50 μm.