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. 2016 Aug 26;29(12):1532–1540. doi: 10.1038/modpathol.2016.144

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Copyright © 2016 The Author(s)

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Immunohistochemical and mutational analysis of sclerosing rhabdomyosarcomas. Representative images of immunohistochemical analysis of MYOD1, myogenin, and MYOD1 (L122R) mutation in sclerosing rhabdomyosarcomas. Upper panel: Case 1. Sclerosing rhabdomyosarcoma (RMS) (I–III). Round to oval cells in a pseudovascular- and cord-like patterns in a dense hyalinized stroma. Hematoxylin and Eosin (H and E), x200. II. Diffuse MYOD1-positive immunostaining. Diaminobenzidine, x400. III. Focal myogenin-positive immunostaining. Diaminobenzidine, x400. Lower panel: Sanger sequencing chromatogram of MYOD1 (L122R, T>G or T>TG) is shown with reference sequences and mutation showing forward and reverse sequencing reads. Arrow indicates nucleotide position that harbors mutation.