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. 2016 Aug 26;29(12):1532–1540. doi: 10.1038/modpathol.2016.144

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Copyright © 2016 The Author(s)

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Immunohistochemical and mutational analysis of spindle cell rhabdomyosarcomas. Representative images of immunohistochemical analysis of MYOD1, myogenin, and MYOD1 (L122R) mutation in spindle cell rhabdomyosarcomas. Upper panel: Case10. Spindle cell rhabdomyosarcoma (I–III). I. Spindle-shaped sarcomatous cells arranged in long intersecting fascicles. Hematoxylin and Eosin (H and E) x200. II. Diffuse MYOD1-positive immunostaining. Diaminobenzidine, x400. III. Myogenin-positive immunostaining. Diaminobenzidine, x400. Lower panel: Sanger sequencing chromatogram of MYOD1 (L122R, T>G or T>TG) is shown with reference sequences and mutation showing forward and reverse sequencing reads. Arrow indicates nucleotide position that harbors mutation.