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. 2016 Aug 26;29(12):1532–1540. doi: 10.1038/modpathol.2016.144

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Copyright © 2016 The Author(s)

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Immunohistochemical and mutational analysis of a sclerosing rhabdomyosarcoma with focal areas of spindle cell rhabdomyosarcoma. Representative images of immunohistochemical analysis of MYOD1, myogenin, and MYOD1 (L122R) mutation in sclerosing rhabdomyosarcoma with focal spindle cell rhabdomyosarcomas. Case 2. Sclerosing rhabdomyosarcoma with focal spindle cells (I–III). I. Oval- to spindle-shaped cells in a dense hyalinized stroma. Hematoxylin and Eosin (H and E) x400. II. Diffuse MYOD1-positive immunostaining. Diaminobenzidine, x400. III. Focal myogenin-positive immunostaining. Diaminobenzidine, x400. Lower panel: Sanger sequencing chromatogram of MYOD1 (L122R, T>G or T>TG) is shown with reference sequences and mutation showing forward and reverse sequencing reads. Arrow indicates nucleotide position that harbors mutation.