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. 2016 Jun 28;19(4):349–357. doi: 10.1038/pcan.2016.21

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Quantitative reverse transcription-PCR (RT-PCR) analysis of c-MYC oncogene in (a) PC3 and (b) DU145 cells after treatment with HCI-2509 for 4 and 24 h. Each replicate was normalized to the reference gene (S18) and induction was calculated relative to the vehicle control. The relative expression of c-MYC mRNA in various conditions was plotted in the form of bar graphs and relative inhibition on treatment with HCI-2509 was tested for significance using the unpaired t-test (GraphPad Prism). Assays were performed in quadruplicate and error bars indicate the standard deviation.